Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics

• Mamta Kumari,
• Amitabha Acharya and
• Praveen Thaggikuppe Krishnamurthy

Beilstein J. Nanotechnol. 2023, 14, 912–926, doi:10.3762/bjnano.14.75

• antibodies (mAbs), and nucleic acid-based materials for targeted drug delivery, have been approved by the Food and Drug Administration (FDA) for the treatment of cancer, arthritis, asthma, psoriasis, pemphigus vulgaris, and chronic urticaria [8]. Antibodies are the primary homing ligands in tumor-targeted

The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy

• Yao Yao,
• Yeongun Ko,
• Grant Grasman,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30

• , payload retention, and interparticle variability in the context of nucleic acid-based nanotherapeutics. In order to illustrate these issues and concerns, we analyzed the materials and methods sections of 50 papers published within the last five years on the topic of NP-mediated delivery of plasmid DNA

• the practices used to determine small-molecule dosing become confounded if applied to nucleic acid payloads (such as plasmids). Direct mass determination of the internalized nucleic acid therapeutics (NATs) is not straightforward and is further complicated given that dosing in these systems should be

• escape. NAT are difficult to quantify if encapsulated into NP delivery carriers. The most common forms of nucleic acid measurement (i.e., UV–vis and fluorescence) provide limited insights for the characterization of NP-encapsulated NATs. Labeling and destructive methods both have drawbacks, ranging from

Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer

• Filip Gorachinov,
• Fatima Mraiche,
• Diala Alhaj Moustafa,
• Ola Hishari,
• Yomna Ismail,
• Jensa Joseph,
• Maja Simonoska Crcarevska,
• Marija Glavas Dodov,
• Nikola Geskovski and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23

• optimize the systems for different target sites, which is especially promising for nucleic acid delivery [96]. One example of multifunctional, multilayer, bioresponsive lipid polymer nanoparticles with a cleavable layer as a vessel for the co-delivery of erlotinib and bevacizumab was recently published by

• nucleic acid co-delivery for the treatment of resistant lung cancer will be discussed below. The challenge of nucleic acid tumor targeting Silencing target genes using siRNA is an attractive therapeutic approach with significant translational potential in lung cancer treatment. The high specificity of

• release are crucial to maximize the delivery or co-delivery of active agents to the site of action in the cell [141]. State-of-the-art LNPs for siRNA gene silencing, that is, stable antisense–lipid particles (SALPs) and stable nucleic acid–lipid particles (SNALPs), were recently developed as PEGylated

Cyclodextrins as eminent constituents in nanoarchitectonics for drug delivery systems

• Makoto Komiyama

Beilstein J. Nanotechnol. 2023, 14, 218–232, doi:10.3762/bjnano.14.21

• resultant well-organized orientation of functional groups in CyD-based nanoarchitectures, should be very appropriate to yield both stable encapsulation of nucleic acid drugs and their prompt release when needed. 3.1 CyD-based nanoarchitectures for delivery of siRNA The therapeutic nucleic acid siRNA is

• delivered to a target site, the therapy should be more efficient than a unimodal therapy. For such a co-delivery, CyD-based nanoarchitectures are very convenient since well-defined structures of CyDs allow for the precise molecular design of nanoarchitectures in which the desired nucleic acid drugs are

• release the siRNA and the antisense DNA strands. The two nucleic acid drugs cooperatively suppressed the expression of the target tumor gene. With a similar strategy, a gene editing machine composed of Cas9 and single guide RNA (sgRNA) [69][70] was delivered to tumors simultaneously with antisense DNA

Single-step extraction of small-diameter single-walled carbon nanotubes in the presence of riboflavin

• Polina M. Kalachikova,
• Anastasia E. Goldt,
• Eldar M. Khabushev,
• Timofei V. Eremin,
• Timofei S. Zatsepin,
• Elena D. Obraztsova,
• Konstantin V. Larionov,
• Liubov Yu. Antipina,
• Pavel B. Sorokin and
• Albert G. Nasibulin

Beilstein J. Nanotechnol. 2022, 13, 1564–1571, doi:10.3762/bjnano.13.130

• biocompatibility of nucleic acids can support biomedical applications of such dispersions. Unfortunately, an extensive ultrasonic treatment required to obtain a dispersion of individual nanotubes might destroy fragile nucleic acid molecules so that their applications are somewhat inhibited. Flavin compounds are

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

• Abu Hashem,
• M. A. Motalib Hossain,
• Ab Rahman Marlinda,
• Mohammad Al Mamun,
• Khanom Simarani and
• Mohd Rafie Johan

Beilstein J. Nanotechnol. 2022, 13, 1458–1472, doi:10.3762/bjnano.13.120

• peptide nucleic acid (PNA) probes [64]. Methylene blue is a very prominent intercalator for DNA-based sensors and biosensors [31][65][66][67][68][69]. For example, Plaxco's group reported the preparation of electrode-immobilised methylene-blue-modified oligonucleotides for electrochemical DNA and aptamer

Studies of probe tip materials by atomic force microscopy: a review

• Ke Xu and
• Yuzhe Liu

Beilstein J. Nanotechnol. 2022, 13, 1256–1267, doi:10.3762/bjnano.13.104

• tool for biomedical research and food detection. Bifunctional probe Yang et al. [54] prepared a novel bifunctionalized colloidal gold nanoprobe and investigated its specificity due to the excellent performance of bifunctional probes for food pathogen detection and nucleic acid analysis. A

• coupling another specific H1N1 oligonucleotide fragment using magnetic microspheres as solid-phase support; both were bound to the target DNA (exact match DNA) to form a colorless nucleic acid probe. The two are combined with the target DNA (exact match DNA) to form a colorless capture probe-target DNA

• only one base mismatch. This study developed a novel self-assembled bifunctionalized colloidal gold nanoprobe with a simple preparation process with high sensitivity and high specificity. This colloidal gold nanoprobe will have a wide range of applications in nucleic acid analysis, especially in

Design of surface nanostructures for chirality sensing based on quartz crystal microbalance

• Yinglin Ma,
• Xiangyun Xiao and
• Qingmin Ji

Beilstein J. Nanotechnol. 2022, 13, 1201–1219, doi:10.3762/bjnano.13.100

• (e.g., DNA analysis, microorganism assays, nucleic acid detection, pharmaceutical substance detection, and gas monitoring) and also a powerful tool for chiral recognition [23][24][25]. The sensitivity and specificity of QCM-based chiral sensors largely depend on the recognition layers on the surface of

Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration

• Se-Kwon Kim,
• Sesha Subramanian Murugan,
• Pandurang Appana Dalavi,
• Sebanti Gupta,
• Sukumaran Anil,
• Gi Hun Seong and
• Jayachandran Venkatesan

Beilstein J. Nanotechnol. 2022, 13, 1051–1067, doi:10.3762/bjnano.13.92

• regeneration, and other applications. Furthermore, chitosan-containing polymer composites are being extensively explored for drug delivery in targeted tumour treatment and nucleic acid delivery in genetic engineering applications. More research is required to optimise chitosan composites utilised in scaffolds

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

• Nathalie B. F. Almeida,
• Thiago A. S. L. Sousa,
• Viviane C. F. Santos,
• Camila M. S. Lacerda,
• Thais G. Silva,
• Rafaella F. Q. Grenfell,
• Flavio Plentz and
• Antero S. R. Andrade

Beilstein J. Nanotechnol. 2022, 13, 873–881, doi:10.3762/bjnano.13.78

• , and specific virus identification. However, these techniques require professional expertise and expensive laboratory equipment and reagents. These conditions are frequently unavailable in endemic regions [8][9][10]. Nucleic acid aptamers are single-strand oligonucleotides (ssDNA or ssRNA) that can

• , high-affinity nucleic acid aptamers have been developed for a wide variety of targets, such as proteins, peptides, viruses, and bacteria [11][12]. Generally, nucleic acid aptamers are developed in vitro by a molecular evolution process based on iterative selection–amplification steps known as

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• molecular weight (25 kDa) [19]. Polyethyleneimine, especially branched 25 kDa PEI, has been accepted as the golden standard for non-viral nucleic acid delivery, providing efficient binding to the cell surface, endosomal release of the cargo, and translocation to the nucleus [25][28][29][30]. To develop

Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications

• Sepand Tehrani Fateh,
• Lida Moradi,
• Elmira Kohan,
• Michael R. Hamblin and
• Amin Shiralizadeh Dezfuli

Beilstein J. Nanotechnol. 2021, 12, 808–862, doi:10.3762/bjnano.12.64

• intracellular delivery of fluorescently labeled mRNA (≈950 kDa) into the colon of healthy C57BL/6 mice using low-frequency US (40 kHz for 0.5 s). Confocal microscopy showed that the mRNA was safely delivered into the colonic mucosa and the colon tissue of mice, in which the US-mediated delivery of the nucleic

• acid was administered, had levels of bioluminescence 11-fold higher than the colon tissue of mice that received mRNA alone. This was suggested to be caused by US-induced cavitation, creating transient pores in the plasma membrane which facilitated the cellular diffusion of macromolecules [87]. In

The nanomorphology of cell surfaces of adhered osteoblasts

• Christian Voelkner,
• Mirco Wendt,
• Regina Lange,
• Max Ulbrich,
• Martina Gruening,
• Susanne Staehlke,
• Barbara Nebe,
• Ingo Barke and
• Sylvia Speller

Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20

• , respectively. An analysis of a single time traces is shown in Figure 10a. Membrane fluctuation amplitudes turn out to amount to a few tens of nanometers and appear to be substantially larger on living than on fixed cells. Since PFA, via denaturation, only stiffens proteins and leaves nucleic acid, lipids, and

Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

• Hiroaki Komuro,
• Masahiro Yamazoe,
• Kosuke Nozaki,
• Akiko Nagai and
• Tetsuo Sasano

Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150

• medical and dental applications, such as dental implants, orthopedics, and drug delivery systems, since it has similar elements found in bone and teeth. In addition, CaP stabilizes the nucleic acid against nuclease degradation, forms ionic interactions with the phosphates of DNA, and its biodegradation is

Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements

• Maria Suciu,
• Corina M. Ionescu,
• Alexandra Ciorita,
• Septimiu C. Tripon,
• Dragos Nica,
• Hani Al-Salami and
• Lucian Barbu-Tudoran

Beilstein J. Nanotechnol. 2020, 11, 1092–1109, doi:10.3762/bjnano.11.94

• with polyethyleneimine and conjugated with CD44 siRNA can be used as non-viral gene therapy vectors. Polyethylene imine is the material of choice for nucleic acid intracellular delivery, as it gives a positive charge to the nanoparticle surface [41]. Polyvinyl alcohol (PVA)-coated SPIONs were tested as

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

• multilayer capsules as drug delivery vehicles [17]. They are capable of encapsulating all kinds of substances and/or molecules ranging from enzymes, nucleic acid, peptides, proteins, therapeutic drugs, biomolecules, fluorescent molecules and nanoparticles (NPs) in their hollow cavity [18]. This can be

Molecular architectonics of DNA for functional nanoarchitectures

• Debasis Ghosh,
• Lakshmi P. Datta and
• Thimmaiah Govindaraju

Beilstein J. Nanotechnol. 2020, 11, 124–140, doi:10.3762/bjnano.11.11

• -functionalized iron oxide nanoparticle system was capable of selectively targeting the cancer cells and, potentially, to act as an MRI contrast agent. The programmability of the DNA tetrahedrons provided an opportunity to conjugate other functional nucleic acid sequences, viz., DNA, siRNA, or DNAzymes, to serve

• demonstrated the design and potential application of SFM-supported DNA nanoarchitectures in sensors and bio-optoelectronics. In another study, we reported a molecular architectonic of adenine (A)- and thymine (T)-appended naphthalenediimide derivatives (NDI-AA and NDI-TT, respectively), with peptide nucleic

• acid (PNA) dimers (clamps) via WC and Hoogsteen base pairing interactions (Figure 6) [75]. The hydrogen bonding interactions, along with hydrophobic interactions, imparted by the nucleobases and the NDI core, facilitated the formation of versatile nano- and microarchitectures. The morphological

Internalization mechanisms of cell-penetrating peptides

• Ivana Ruseska and
• Andreas Zimmer

Beilstein J. Nanotechnol. 2020, 11, 101–123, doi:10.3762/bjnano.11.10

• contrast to the common cationic CPPs, Arukuusk et al. [88] suggest negatively charged CPPs as oligonucleotide carriers. The CPPs are not inherently anionic, but confer a negative charge after they are complexed with a nucleic acid in cell culture media. The group has already demonstrated that the uptake of

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• ). Specifically, siRNA-loaded nanoparticles were incubated in the presence of RNAse I at concentrations sufficiently high to degrade partially (at 0.5 U) or completely (at 5 U) the same amount of non-encapsulated nucleic acid (labelled as “free” in Figure 4). RNA was then decomplexed by enzymatically digesting

• enzymatic reaction was allowed to occur for 3 h. Finally, 4.7 µL of a solution of heparin (200 mg/mL in nuclease-free water; corresponding approximately to a z-Hep/z-siRNA ratio of 250) were added. The resulting mixture was incubated overnight at 25 °C. After centrifugation (13,000 rpm, 30 min), the nucleic

• acid released in solution was quantified using polyacrylamide gel electrophoresis (PAGE, 18-well/30 µL, 15% Criterion TM TBE-Urea Gel, Biorad; 70 min, 120 V). Gels were imaged with a UV trans-illuminator (ChemiDoc™ MP System #170-8280) adjusting the exposure time to avoid saturation, and the acquired

Long-term stability and scale-up of noncovalently bound gold nanoparticle-siRNA suspensions

• Anna V. Epanchintseva,
• Julia E. Poletaeva,
• Dmitrii V. Pyshnyi,
• Elena I. Ryabchikova and
• Inna A. Pyshnaya

Beilstein J. Nanotechnol. 2019, 10, 2568–2578, doi:10.3762/bjnano.10.248

• comparison, we presented the electropherogram of the initial bare gold nanoparticles (Figure 1G). The density of the siRNA molecules on the AuNP surface is an indicator of the efficiency of the nucleic acid transporter: the higher the density, the less amount of the preparation can be administered, thus

Review of advanced sensor devices employing nanoarchitectonics concepts

• Katsuhiko Ariga,
• Tatsuyuki Makita,
• Masato Ito,
• Taizo Mori,
• Shun Watanabe and
• Jun Takeya

Beilstein J. Nanotechnol. 2019, 10, 2014–2030, doi:10.3762/bjnano.10.198

• biomolecules including amino acids [156], peptides [157][158][159], sugars [160][161], nucleic acid bases [162][163], and nucleotides [164][165][166] at well-designed interfacial environments. In order to design and fabricate sensors with better performance, interfacial nanoarchitectonics should be crucial

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

• Barbara Pem,
• Igor M. Pongrac,
• Lea Ulm,
• Ivan Pavičić,
• Valerije Vrček,
• Darija Domazet Jurašin,
• Marija Ljubojević,
• Adela Krivohlavek and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175

• , Hamburg, Germany). After treatment with AgNPs or AuNPs (at 5 mg Ag/Au L−1), the cells were washed with PBS, fixed with ice-cold methanol (−20 °C), and stained with F-actin-phalloidin and Hoechst 33258 nucleic acid dyes. The slides were then mounted in Fluoroshield Antifade Mounting Medium and their images

• with phalloidin to stain actin and visualize cell cytoskeleton (green), nucleic acid staining using Hoechst 33258 fluorescent dye (blue) and CLSM reflectance signals (red). The overlay of fluorescence stains and segmented reflectance signals are given in (g,h). The control cells show no high intensity

Materials nanoarchitectonics at two-dimensional liquid interfaces

• Katsuhiko Ariga,
• Michio Matsumoto,
• Taizo Mori and
• Lok Kumar Shrestha

Beilstein J. Nanotechnol. 2019, 10, 1559–1587, doi:10.3762/bjnano.10.153

• ]. Although molecular recognition via hydrogen bonding are quite difficult in a highly polar aqueous media, the molecular recognition of sugars [145][146], peptides [147][148][149], amino acids [150], nucleic acid bases [151][152], and nucleotides [153][154] is accomplishable at the air–water interface even

Non-agglomerated silicon–organic nanoparticles and their nanocomplexes with oligonucleotides: synthesis and properties

• Asya S. Levina,
• Marina N. Repkova,
• Nadezhda V. Shikina,
• Zinfer R. Ismagilov,
• Svetlana A. Yashnik,
• Dmitrii V. Semenov,
• Yulia I. Savinovskaya,
• Natalia A. Mazurkova,
• Inna A. Pyshnaya and
• Valentina F. Zarytova

Beilstein J. Nanotechnol. 2018, 9, 2516–2525, doi:10.3762/bjnano.9.234

• "Vector", Koltsovo, Novosibirsk region, 630559, Russia 10.3762/bjnano.9.234 Abstract The development of efficient and convenient systems for the delivery of nucleic-acid-based drugs into cells is an urgent task. А promising approach is the use of various nanoparticles. Silica nanoparticles can be used as

• vehicles to deliver nucleic acid fragments into cells. In this work, we developed a method for the synthesis of silicon–organic (Si–NH2) non-agglomerated nanoparticles by the hydrolysis of aminopropyltriethoxysilane (APTES). The resulting product forms a clear solution containing nanoparticles in the form

• ; non-agglomerated silicon–organic nanoparticles; penetration; Si–NH2·ODN nanocomplexes; Introduction The development of efficient and convenient systems for the delivery of nucleic-acid-based drugs into cells is an urgent task. The solution to this problem would allow for the use of these drugs in

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• this assay, drug-induced apoptosis was confirmed by CaP@5-FU NP treatment. Hoechst 33342 and rhodamine B Apoptotic cells show specific characteristic features such as morphological changes, nuclear fragmentation, and cytoplasmic constriction [41]. The nucleic acid specific dye Hoechst 33342 and the

• propidium iodide staining for DNA quantification and to unveil the different cell cycle phases. Propidium iodide (PI), a fluorogenic dye, intercalates with nucleic acid in a stoichiometric fashion and can be used as an effective indicator dye in cellular DNA quantification with flow cytometry [47]. In

• track the morphological changes occurring in the cells after treatment with CaP@5-FU NPs in a time-dependent manner. Hoechst 33342 can effectively stain the nucleic acid and specifically identify changes in the nucleus, whereas rhodamine B stains the cytoplasmic vesicles uniformly, enabling the